Berkeley Lab - California - Lawrence Berkeley National Lab’s ( ), has an opening for a Plant Biology Research Assistant to work as part of the Feedstocks Division of the Joint BioEnergy Institute (JBEI) under direct supervision of Dr. Henrik V. Scheller and in close collaboration with Dr. Jenny Mortimer to assist in maintaining and characterizing plants. The Research Assistant will assist with growing plants in the greenhouse and in growth chambers. Samples will be taken from these plants as well as from plants grown in the field by collaborators. Plant samples will be analyzed through genotyping by PCR, real-time RT-PCR, and determination of plant cell wall sugar composition by high performance anion exchange chromatography. Samples will also be prepared for RNAseq and metabolomics carried out by collaborators. What You Will Do: Support experiments using standard techniques of molecular biology, including extraction and manipulation of DNA and RNA, gel electrophoresis, PCR, and qPCR Support experiments for cell wall analysis, including extraction of cell wall material, chemical and enzymatic hydrolysis, and analysis by HPLC. Maintain plants, including planting and harvesting seeds Prepare media and maintain plant cell cultures Genotype mutant and transgenic plants Maintain well-documented laboratory notebook Assist with day-to-day lab management activities such as ordering, inventory maintenance, and waste management What is Required: Bachelor’s degree in Molecular Biology, Biochemistry, or similar field preferred, or equivalent work experience Experience with basic molecular biology techniques, including PCR, gel electrophoresis, sterile culturing, and preparation and analysis of DNA Knowledge of underlying principles of biochemistry and molecular biology methods Experience with using equipment such as pH meters, autoclaves, balances, etc. Excellent organizational and multi-tasking skills Attention to detail and careful record keeping skillsAbility to follow well-defined protocols to produce consistent reproducible results Interest in and ability to learn and implement new experimental techniques Strong interpersonal and communications skills will the ability to work as part of a larger team Driving license (necessary to drive to greenhouse and field sites) Desired Qualifications: Experience with plants and preparing samples from different tissues Experience with using equipment such as HPLC, LC-MS/MS, or GC-MS Experience with biochemical techniques, e.g., SDS-PAGE, Western blot, enzyme assays and chromatography Experience handling microbes such as Agrobacterium, E. coli and yeast For full consideration, please apply by March 17, 2021. Notes: This is a full time 1 year and 6 months, term appointment with the possibility of extension or conversion to Career appointment based upon satisfactory job performance, continuing availability of funds and ongoing operational needs. This position is represented by a union for collective bargaining purposes. Salary will be determined based on range by collective bargaining agreement. This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment. Work will be primarily performed at: EmeryStation East — Bldg. 978, 5885 Hollis St., 4th floor, Emeryville, CA. If you have any questions, feel free to contact Henrik Schellerat HScheller@lbl.gov. Learn about us! Environmental Genomics and Systems Biology Mission: Linking genome biology to ecosystem dynamics. Vision: Sustainable solutions to energy and environmental challenges developed through mechanistic understanding of ecosystem dynamics. Approach: We develop systems-level models using integrated molecular observation and controlled manipulation of model organisms and defined biomes to design and test interventions that promote beneficial outcomes. Berkeley Lab (LBNL) addresses the world’s most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab’s scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy’s Office of Science. Working at Berkeley Lab has many rewards including a competitive compensation program, excellent health and welfare programs, a retirement program that is second to none, and outstanding development opportunities. To view information about the many rewards that are offered at Berkeley Lab- . Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the under 41 CFR 60-1.4. Click to view the poster and supplement: "Equal Employment Opportunity is the Law. Lawrence Berkeley National Laboratory encourages applications from women, minorities, veterans, and other underrepresented groups presently considering scientific research careers.... - Permanent - Full-time
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