Research Assistant

Berkeley Lab - California - Lawrence Berkeley National Lab's ( ), has an opening for a Research Assistant in the research group of Dr. Aindrila Mukhopadhyay at Emery Station East. The Research Assistant will take part in ongoing work funded by the . This project seeks to address the important and challenging task of developing new microbial hosts for biofuel production. The Research Assistant will directly contribute to bench-level experimental research involved in microbial strain engineering and optimization to unlock the potential of renewable energy and other biologically derived materials from single cell organisms, such as Pseudomonas putida. What You Will Do: DNA design, manipulation, and cloning. Engineering of bacterial strains such as Pseudomonas putida, Corynebacterium glutamicum, and Escherichia coli with classical and next generation editing methods, such as homologous recombination and CRISPR. Functional expression and evaluation of candidate pathway genes in microbial hosts, especially in the context of Sartorius 2L bioreactor scale-up systems (and similar). Growing, manipulating and archiving bacterial cultures. Metabolite and proteomic sample prep for LC- and GC-MS. Additional Responsibilities as needed: Data recording and report preparation for supervising scientists. Participating in community laboratory jobs at the RA level. (preparation of antibiotics, buffer/media preparation) Miscellaneous tasks required to ensure a functional research team. (preparing competent cells, ordering supplies, etc.) What is Required: B.S. degree in microbial or molecular biology preferred with demonstrated success in applying molecular biology research techniques. Working experience in a fast-paced biotechnology startup environment. (preferably at least six months) Experience in the following techniques: PCR; DNA sequence analysis; DNA isolation, gene cloning and expression; genetic manipulation and cultivation of E. coli or Pseudomonas putida. Familiarity with recent cloning methods. (Gibson, HiFi assembly, Cas9/CRISPR etc.) Strong analytical and communication skills. Ability to be cooperative, team-oriented, mission-oriented, and maintain an up-to-date lab notebook. Must adhere to all campus and EH&S safety policies and model safe lab best practices for others. Additional Desired Qualifications: Experience with bacterial strain growth and genetics in Pseudomonas putida Familiarity with Sartorius Biostat B bioreactors or Sartorius Ambr250 automated bioreactors Familiarity with ssDNA oligo recombineering a plus. The posting shall remain open until the position is filled. Notes: This is a full time, 1-year term appointment with the possibility of extension or conversion to Career appointment based upon satisfactory job performance, continuing availability of funds and ongoing operational needs. M-F, Non-exempt (hourly paid) from overtime pay. This position is represented by a union for collective bargaining purposes. Salary will be determined based on range by collective bargaining agreement. This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment. Work will be primarily performed at: Joint BioEnergy Institute (JBEI) - Bldg. 978, 5885 Hollis St., 4th floor, Emeryville, CA. Learn about us! Biological Systems and Engineering Division Vision: To lead efforts that combine the power of biology with the tools of engineering to develop sustainable energy and biomanufacturing solutions and improve human health. Mission: Advance a mechanistic and predictive understanding of complex biological systems over multiple scales in terms of their responses to manipulation, stress, disease and environmental challenges and translate this knowledge using engineering principles to develop resilient systems, tools, and processes for the efficient production of fuels, chemicals, materials, tissues, and therapeutics. Berkeley Lab (LBNL) addresses the world's most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab's scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy's Office of Science. Working at Berkeley Lab has many rewards including a competitive compensation program, excellent health and welfare programs, a retirement program that is second to none, and outstanding development opportunities. To view information about the many rewards that are offered at Berkeley Lab- . Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the under 41 CFR 60-1.4. Click to view the poster and supplement: "Equal Employment Opportunity is the Law. Lawrence Berkeley National Laboratory encourages applications from women, minorities, veterans, and other underrepresented groups presently considering scientific research careers.... - Permanent - Full-time

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